a2z-chromatin/a2z-methylation

Authors: Travis Wrightsman

License: MIT

Contributed by: Travis Wrightsman

Cite as: https://doi.org/10.1101/2021.11.11.468292

Type: None

Postprocessing: None

Trained on: ATAC-Seq data from 12 angiosperms and UMR calls from 10 angiosperms

Source files

Model predicting chromatin accessibility or lack of DNA methylation from Angiosperm DNA sequence

Create a new conda environment with all dependencies installed

kipoi env create a2z-chromatin
source activate kipoi-a2z-chromatin

Test the model

kipoi test a2z-chromatin/a2z-methylation --source=kipoi

Make a prediction

kipoi get-example a2z-chromatin/a2z-methylation -o example
kipoi predict a2z-chromatin/a2z-methylation \
  --dataloader_args='{"intervals_file": "example/intervals_file", "fasta_file": "example/fasta_file"}' \
  -o '/tmp/a2z-chromatin|a2z-methylation.example_pred.tsv'
# check the results
head '/tmp/a2z-chromatin|a2z-methylation.example_pred.tsv'

Create a new conda environment with all dependencies installed

kipoi env create a2z-chromatin
source activate kipoi-a2z-chromatin

Get the model

import kipoi
model = kipoi.get_model('a2z-chromatin/a2z-methylation')

Make a prediction for example files

pred = model.pipeline.predict_example(batch_size=4)

Use dataloader and model separately

# Download example dataloader kwargs
dl_kwargs = model.default_dataloader.download_example('example')
# Get the dataloader and instantiate it
dl = model.default_dataloader(**dl_kwargs)
# get a batch iterator
batch_iterator = dl.batch_iter(batch_size=4)
for batch in batch_iterator:
    # predict for a batch
    batch_pred = model.predict_on_batch(batch['inputs'])

Make predictions for custom files directly

pred = model.pipeline.predict(dl_kwargs, batch_size=4)

Get the model

library(reticulate)
kipoi <- import('kipoi')
model <- kipoi$get_model('a2z-chromatin/a2z-methylation')

Make a prediction for example files

predictions <- model$pipeline$predict_example()

Use dataloader and model separately

# Download example dataloader kwargs
dl_kwargs <- model$default_dataloader$download_example('example')
# Get the dataloader
dl <- model$default_dataloader(dl_kwargs)
# get a batch iterator
it <- dl$batch_iter(batch_size=4)
# predict for a batch
batch <- iter_next(it)
model$predict_on_batch(batch$inputs)

Make predictions for custom files directly

pred <- model$pipeline$predict(dl_kwargs, batch_size=4)

Get the docker image

docker pull kipoi/kipoi-docker:sharedpy3keras2tf2-slim

Get the full sized docker image

docker pull kipoi/kipoi-docker:sharedpy3keras2tf2

Get the activated conda environment inside the container

docker run -it kipoi/kipoi-docker:sharedpy3keras2tf2-slim

Test the model

docker run kipoi/kipoi-docker:sharedpy3keras2tf2-slim kipoi test a2z-chromatin/a2z-methylation --source=kipoi

Make prediction for custom files directly

# Create an example directory containing the data
mkdir -p $PWD/kipoi-example 
# You can replace $PWD/kipoi-example with a different absolute path containing the data 
docker run -v $PWD/kipoi-example:/app/ kipoi/kipoi-docker:sharedpy3keras2tf2-slim \
kipoi get-example a2z-chromatin/a2z-methylation -o /app/example 
docker run -v $PWD/kipoi-example:/app/ kipoi/kipoi-docker:sharedpy3keras2tf2-slim \
kipoi predict a2z-chromatin/a2z-methylation \
--dataloader_args='{'intervals_file': '/app/example/intervals_file', 'fasta_file': '/app/example/fasta_file'}' \
-o '/app/a2z-chromatin_a2z-methylation.example_pred.tsv' 
# check the results
head $PWD/kipoi-example/a2z-chromatin_a2z-methylation.example_pred.tsv

Install apptainer

https://apptainer.org/docs/user/main/quick_start.html#quick-installation-steps

Make prediction for custom files directly

kipoi get-example a2z-chromatin/a2z-methylation -o example
kipoi predict a2z-chromatin/a2z-methylation \
--dataloader_args='{"intervals_file": "example/intervals_file", "fasta_file": "example/fasta_file"}' \
-o 'a2z-chromatin_a2z-methylation.example_pred.tsv' \
--singularity 
# check the results
head a2z-chromatin_a2z-methylation.example_pred.tsv

Schema

Inputs

Single numpy array

Name: None

Shape: (600, 4)

Doc: one-hot encoded DNA sequence

Targets

Single numpy array

Name: None

Shape: (1,)

Doc: is accessible or is unmethylated (DNA), depending on model

Dataloader

Defined as: kipoiseq.dataloaders.SeqIntervalDl

Doc: Dataloader for a combination of fasta and tab-delimited input files such as bed files. The dataloader extracts regions from the fasta file as defined in the tab-delimited `intervals_file` and converts them into one-hot encoded format. Returned sequences are of the type np.array with the shape inferred from the arguments: `alphabet_axis` and `dummy_axis`.

Authors: Ziga Avsec , Roman Kreuzhuber

Type: Dataset

License: MIT

Arguments

intervals_file : bed3+<columns> file path containing intervals + (optionally) labels

fasta_file : Reference genome FASTA file path.

num_chr_fasta (optional): True, the the dataloader will make sure that the chromosomes don't start with chr.

label_dtype (optional): None, datatype of the task labels taken from the intervals_file. Example: str, int, float, np.float32

use_strand (optional): reverse-complement fasta sequence if bed file defines negative strand. Requires a bed6 file

ignore_targets (optional): if True, don't return any target variables

Model dependencies

conda:

python=3.7
cython=0.29
tensorflow=2.6.0
keras=2.6.0
pip=21.2.4
numpy=1.19.5

pip:

kipoiseq

Dataloader dependencies

conda:

bioconda::pybedtools
bioconda::pyfaidx
bioconda::pyranges
numpy
pandas

pip:

kipoiseq